A consensus view of DNA binding by the C family of replicative DNA polymerases
نویسندگان
چکیده
منابع مشابه
A consensus view of DNA binding by the C family of replicative DNA polymerases.
T he DNA polymerases that duplicate bacterial chromosomes are members of the C family of polymerases, which shares no sequence homology with any of the other DNA polymerase families. In Gram-negative bacteria the replicative polymerase is polymerase III -subunit (Pol III), whereas in Gram-positive bacteria the replicative polymerase is called Pol C. Even though the first C family polymerase was...
متن کاملReplication of ribonucleotide-containing DNA templates by yeast replicative polymerases.
The major replicative DNA polymerases of S. cerevisiae (Pols α, δ, and ɛ) incorporate substantial numbers of ribonucleotides into DNA during DNA synthesis. When these ribonucleotides are not removed in vivo, they reside in the template strand used for the next round of replication and could potentially reduce replication efficiency and fidelity. To examine if the presence of ribonucleotides in ...
متن کاملMechanism of strand displacement synthesis by DNA replicative polymerases
Replicative holoenzymes exhibit rapid and processive primer extension DNA synthesis, but inefficient strand displacement DNA synthesis. We investigated the bacteriophage T4 and T7 holoenzymes primer extension activity and strand displacement activity on a DNA hairpin substrate manipulated by a magnetic trap. Holoenzyme primer extension activity is moderately hindered by the applied force. In co...
متن کاملAbundant ribonucleotide incorporation into DNA by yeast replicative polymerases.
Measurements of nucleoside triphosphate levels in Saccharomyces cerevisiae reveal that the four rNTPs are in 36- to 190-fold molar excess over their corresponding dNTPs. During DNA synthesis in vitro using the physiological nucleoside triphosphate concentrations, yeast DNA polymerase epsilon, which is implicated in leading strand replication, incorporates one rNMP for every 1,250 dNMPs. Pol del...
متن کاملPlant organellar DNA polymerases are replicative and translesion DNA synthesis polymerases
Genomes acquire lesions that can block the replication fork and some lesions must be bypassed to allow survival. The nuclear genome of flowering plants encodes two family-A DNA polymerases (DNAPs), the result of a duplication event, that are the sole DNAPs in plant organelles. These DNAPs, dubbed Plant Organellar Polymerases (POPs), resemble the Klenow fragment of bacterial DNAP I and are not r...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2008
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.0811279106